四氮唑比色法
⑩ The absorbance ( A) value in each well was assayed with tetrazoline colorimetry as reflection of proliferation of the chondrocyte.
⑩采用四氮唑蓝比色法检测各孔软骨细胞的A值,以反映软骨细胞增殖情况。
Methods Calvarium osteoblasts of new born SD rat were cultured and exposed to different concentrations of Fructus Psoraleae extract, then the content of alkaline phosphatase ( ALP) in the cell was measured and the cell proliferation was detected by tetrazoline colorimetry ( MTT).
方法在新生大鼠颅骨成骨细胞体系中加入不同浓度的补骨脂提取物,检测细胞内碱性磷酸酶(ALP)含量,并用MTT法检测细胞增殖。
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